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技術支持

全血RNA管和RNA保護液
更新時間:2017-11-30   點擊次數(shù):4595次

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PAXgene® Blood RNA Tube 全血RNA管

適用:血液樣本

 

Ambion® RNAlater™ Stabilization SolutionRNA 保護液、固定液

適用:組織樣品或液氮冷凍樣品

 

PAXgene® Blood RNA Tube 全血RNA管

 

 

 

產(chǎn)品英文名 PAXgene® Blood RNA Tube

產(chǎn)品中文名 PAXgene® 全血RNA管

產(chǎn)品介紹  PAXgene® 全血RNA管能穩(wěn)定血細胞內的RNA水平,提供一份zui接近人體內狀態(tài)的RNA標本,延長血液標本體外放置的時間,利于批量采集,細胞轉錄水平保真性好,可用于與血細胞RNA相關的RT-PCR檢測,人類疾病基因轉錄譜的研究及其他RNA轉錄相關的檢測。特殊RNA穩(wěn)定劑、zui小化體外血RNA降解或基因誘導,延長標本體外放置時間,全血RNA原管室溫保存3~5天,凍存條件下儲存8年。

 

產(chǎn)品訂購信息

品牌

貨號

產(chǎn)品描述

包裝

BD

762165

2.5mL全血RNA管,6.9mL添加劑,紅色安全蓋

100/箱

 

詳細說明

Tube Material            Plastic

Tube Size              16x100 mm

Draw Volume             2.5 mL

Closure Type            BD Hemogard™

Closure Color            Red

Label                Paper

Cell Separator           No

Additive              6.9 mL of Proprietary RNA stabilization additive

Sterilization               Radiation, 10-6 SAL, ISO11137-1

Regulatory Status            IVD

 

Intended Use

The PAXgene® Blood RNA System consists of a blood collection tube (PAXgene® Blood RNA Tube) and nucleic acid purification kit (PAXgene® Blood RNA Kit). It is intended for the collection, storage, and transport of blood and stabilization of intracellular RNA in a closed tube and subsequent isolation and purification of intracellular RNA from whole blood for RT-PCR used in molecular diagnostic testing.

Performance characteristics for the PAXgene® Blood RNA System have only been established with FOS and IL1B gene transcripts. The user is responsible for establishing appropriate PAXgene® Blood RNA System performance haracteristics for other target transcripts.

 

Warnings

1. Contents of this tube are irritating to skin.

• After inhalation, supply fresh air: consult doctor in case of complaints.

• After skin contact, immediay wash with water and soap, and rinse thoroughly.

• After eye contact, rinse opened eye for fifteen minutes under running water, then consult a doctor.

• After swallowing, immediay call a doctor.

A blood collection set must be used with the PAXgene® Blood RNA Tube. See Ordering Information.

 

Limitations

1. Under-filling of the PAXgene® Blood RNA Tubes will result in an incorrect blood-to-additive

ratio and may lead to incorrect analytic results or poor product performance.

2. The PAXgene® Blood RNA System is not suitable for the collection and purification of viral

RNA.

3. Blood samples obtained with the PAXgene® Blood RNA Tube must be prepared only with the PAXgene® Blood RNA Kit. For catalog numbers, see Ordering Information.

4. The quantity of blood drawn should be 2.5mL per PAXgene® Blood RNA Tube, but this volume might vary with altitude, ambient temperature, barometric pressure, tube age, venous pressure, and filling technique.

5. The PAXgene® Blood RNA Kit is intended for purification of intracellular RNA from human whole blood with leukocytes counts between 4.8 x 106- 1.1 x 107leukocytes/mL.

 

Precautions

1. Practice universal precautions. Use gloves, gowns, eye protection, other personal protective equipment, and engineering controls to protect from blood splatter, blood leakage, and potential exposure to bloodborne pathogens.

2. Handle all biologic samples and blood collection sets "sharps" according to the policies and procedures of your facility. Obtain appropriate medical attention in the event of any exposure to biologic samples (for example,through a puncture injury), since they may transmit viral hepatitis, HIV (AIDS), or other infectious diseases.Utilize any built-in needle protector, if the blood collection set provides one. PreAnalytiX does not recommend reshielding used needles. However, the policies and procedures of your facility may differ and must always be followed.

3. Discard all blood collection tubes in biohazard containers approved for their disposal.

4. Do not re-use the PAXgene® Blood RNA Tubes.

5. Do not use the PAXgene® Blood RNA Tubes after the expiration date printed on the tube label.

6. Since the PAXgene® Blood RNA Tube contains a chemical additive, a blood collection set must be used for blood collection to prevent possible backflow from the tube. See Specimen Collection and Preparation for Analysis section.

7. Excessive centrifugation speed (over 10,000 RCF) may cause the PAXgene® Blood RNA Tube breakage, exposure to blood and possible injury.

8. Do not transfer a sample from a syringe to a tube.

 

Storage

Store the unused PAXgene® Blood RNA Tubes at 18ºC to 25ºC. Limited excursion temperatures up to 40ºC are permitted.

 

762165 現(xiàn)貨歡迎您致電 華雅再生醫(yī)學旗艦公司:紅榮微再(上海)生物工程技術有限公司  :1500 1904 520。紅榮微再-客服: 2395557778  經(jīng)銷商專員

Ambion® RNAlater™ Stabilization Solution RNA保護液

 

 

 

 

 

詳細介紹

產(chǎn)品英文名 Ambion® RNAlater™ Stabilization Solution

產(chǎn)品中文名 Ambion® RNAlater™ RNA 固定液

產(chǎn)品介紹  Ambion® RNAlater™ Stabilization Solution: RNA固定液為水溶性、無組織毒性的保存試劑,可快速滲透到組織中,固定并保護細胞RNA。RNAlater® 固定并保護未破壞的未冷凍組織樣品中的細胞RNA,無需為后續(xù)處理而快速處理組織樣品或液氮冷凍樣品。組織塊可獲得并浸入RNAlater® 中進行保存,不影響后續(xù)RNA分離所獲得的RNA數(shù)量和質量。RNAlater® 可加到細胞團甚至培養(yǎng)基中的細胞中,然后樣品即可冷凍或不冷凍保存。

 

Ambion® RNAlater™ Stabilization Solution訂購信息

 

品牌

貨號

產(chǎn)品描述

包裝

單價

Thermo

AM7020

Ambion® RNAlater™ Stabilization Solution

RNA 保護液、固定液

100 mL

1422元

Thermo

AM7021

Ambion® RNAlater™ Stabilization Solution

RNA 保護液、固定液

500 mL

4444元

 

 

RNAlater™ Stabilization Solution RNA 保護液特點

• 一種試劑即可迅速滅活RNA酶,穩(wěn)定組織或細胞中的RNA

• 無需液氮冷凍樣品或將樣品快速轉入實驗室冰箱中

• 多數(shù)組織樣品無需冷凍和研磨

• “野外”組織采集的理想選擇

• RNA在 37°C 下可穩(wěn)定1天, 25°C 下1周, 4°C 下1個月, -20°C 下無限期。

• 與包括大多數(shù)RNA分離試劑盒在內的多數(shù)RNA分離程序相兼容

 

RNA保護液上下游產(chǎn)品

 

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產(chǎn)品描述

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Thermo

4444436

TaqMan Fast Virus 1-Step Master Mix 酶

10mL/瓶

Thermo

AM9920

DEPC-Treated Water DEPC處理水

500mL/瓶

Thermo

15567027

UltraPure 1M pH7.5 Tris-HCI Buffer緩沖液

1 L/瓶

Thermo

AM9262

EDTA (0.5 M), pH 8.0 溶液

1000mL/瓶

Thermo

AM2222

Ambion DNase I (RNase-free) 酶

2000U/支

Thermo

12574026

SuperScriptIII One-Step RT-PCR含Taq DNA 酶

100次/盒

QIAGEN

57704

QIAamp MinElute Virus Spin Kit(50)試劑盒

50次/盒

Promega

P2077

T7 RNA Polymerase 聚合酶

5000U/支

Corning

21-031-CVR

DPBS(1x)緩沖液(不含鈣鎂)

500mL/瓶

NEB

M0363L

T5 核酸外切酶

5000U/組

 

歡迎您致電 華雅再生醫(yī)學旗艦公司:紅榮微再(上海)生物工程技術有限公司  :1500 1904 520。紅榮微再-客服: 2395557778  經(jīng)銷商專員

 

 

 

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