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AB血清對(duì)人體外終末期紅系分化進(jìn)程的影響

摘要

目的：探究體外誘導(dǎo)造血干細(xì)胞向紅系分化過(guò)程中人AB血清對(duì)終末期紅系分化進(jìn)程的影響。

方法：從臍帶血中用免疫磁珠法分離提取CD34+細(xì)胞，體外誘導(dǎo)其向紅系分化。根據(jù)培養(yǎng)體系中是否添加人AB血清，設(shè)置實(shí)驗(yàn)組為添加人AB血清組，對(duì)照組為不添加人AB血清組。通過(guò)檢測(cè)細(xì)胞表面紅系分化標(biāo)志分子糖化血紅蛋白A(glycophorin A,GPA)、人紅細(xì)胞陰離子交換蛋白Band3及整合素α4(α4-integrin)的表達(dá)反映終末紅系分化進(jìn)程,用流式細(xì)胞術(shù)檢測(cè)紅系細(xì)胞終末分化脫核情況。結(jié)果:與對(duì)照組相比,實(shí)驗(yàn)組GPA陽(yáng)性細(xì)胞百分率較高,Band3表達(dá)較高,而α4-integrin表達(dá)較低(P<0.05)。實(shí)驗(yàn)組細(xì)胞脫核百分率明顯高于對(duì)照組(P<0.05)。結(jié)論:在體外紅系發(fā)育培養(yǎng)體系中,人AB血清對(duì)終末期紅系分化進(jìn)程有一定的促分化、促脫核作用。

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2016 Dec 28;41(12):1245-1251. doi: 10.11817/j.issn.1672-7347.2016.12.001.

[Effect of AB serum on human terminal erythroid differentiation ex vivo].

[Article in Chinese; Abstract available in Chinese from the publisher]

Liao J1,et al.

Abstract in English, Chinese

OBJECTIVE:

To demonstrate the effect of AB serum on terminal erythroid differentiation ex vivo.? Methods: After separation of CD34+ cells from cord blood, the cells were cultured and divided into a control group and an experimental group. The effects of AB serum were examined by the expressions of different markers (GPA, Band3 and α4-integrin) for erythroblast differentiation and enucleation by flow cytometry. Results: The CD34+ cells were successfully differentiated to enucleated red blood cells. There were evident differences among the expressions of GPA, Band3 and α4-integrin between the 2 groups. The percentage of GPA positive cells in the experimental group was bigger than that in the control group in every time point. The expression of Band3 in the experimental group was higher than that in the control group. The expression of α4-integrin in the experimental group was lower than that in the control group. In addition, the enucleation rate in the experimental group was higher than that in the control group.? Conclusion: AB serum can promote the cell differentiation and enucleation during terminal erythroid differentiation in vitro.

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GemCell™人AB血清來(lái)自于美國(guó)FDA認(rèn)證的AB血型健康男性供體。供體AB血漿加抗凝劑經(jīng)去纖維蛋白處理得到AB血清，一致性較高。所有供體的病毒標(biāo)志物檢測(cè)都是陰性。

人AB血清作為牛血清（FBS、FCS）的替代物，不含外源物，適合組織工程、移植和細(xì)胞治療領(lǐng)域的間充質(zhì)干細(xì)胞擴(kuò)增，及其他免疫系統(tǒng)相關(guān)的細(xì)胞培養(yǎng)和反應(yīng)。

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紅榮微再（上海）生物工程技術(shù)有限公司以“傳遞科學(xué)價(jià)值，服務(wù)科學(xué)研究”為宗旨，主營(yíng)：干細(xì)胞、醫(yī)療、細(xì)胞治療、器官再生四大板塊的產(chǎn)品。